目的 研究虎杖(Polygonum cuspidatum Sieb. et Zucc)的化学成分。方法 实验分别以石油醚-乙酸乙酯(9:1、7:1、5:1、3:1、1:1)作为硅胶柱色谱梯度洗脱剂,收集到的组分经制备HPLC进一步分离纯化,1H-NMR、13C-NMR、DEPT等核磁共振谱和质谱的方法对化合物进行结构鉴定。结果 分离并鉴定出8种化合物,分别是:22(Z)-麦角甾-4,6,8,22-四烯-3-酮(1)、(22E,24R)-豆甾-1,4-二烯-3-酮(2)、(E)-乙基十八碳-16-烯酸乙酯(3)、齐墩果酸(4)、大黄素(5)、白藜芦醇(6)、白藜芦醇苷(7)和6'-没食子酸-4-O-D-白藜芦醇苷酯(8)。结论 化合物(1)22(Z)-麦角甾-4,6,8,22-四烯-3-酮为新的天然产物(专利号:ZL 2013 10205435.5),化合物(2)及(3)为虎杖药材中首次发现。
Abstract
OBJECTIVE To study the chemical constituents from Polygonum cuspidatum. METHODS The petroleum ether-ethyl acetate solvents at different ratios of 9:1, 7:1, 5:1, 3:1 and 1:1 were used as gradient eluents for silica gel column chromatography, and samples collected were further isolated and purified by preparative high performance liquid chromatography. Their structures were elucidated by physico-chemical constants and spectroscopic methods. RESULTS Eight compounds were obtained and identified as 22(Z)-ergosterol-4,6,8,22-tetraene-3-one(1), (22E,24R)-stigma-1,4-diene-3-one(2), (E)-ethyl octadecanoic-16-enoic acid ethyl ester(3), oleanolic acid(4), emodin(5), resveratrol(6), trans-resveratrol glycoside(7) and 6'-gallic acid-4-O-D-resveratrol ester(8).CONCLUSION Compound 1 is a new natural compound (patent No. ZL 2013 10205435.5), compound 2 and 3 are isolated from this plant for the first time.
关键词
虎杖 /
化学成分 /
22(Z)-麦角甾-4 /
6 /
8 /
22-四烯-3-酮
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Key words
Polygonum cuspidatum /
chemical constituent /
22 (Z) -ergosterol-4, 6, 8, 22-tetraene-3-one
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中图分类号：
R282
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参考文献
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脚注
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基金
江苏省南通市科研项目资助(MS12017023-6)
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